The DNA Biotechnology Facility is a core laboratory providing services for automated DNA sequencing, DNA isolation, instrumentation, and a reagent Supply Center Program for biology research. All services are available to the entire University of Connecticut community and external academic and industrial customers. The mission of the Facility is to provide expertise and access to capital equipment and associated resources to obtain effective cost sharing and a platform for acquiring new technology. Mr. Xiaoyang Liu heads the Facility and administers the services.
Services and Instrumentation
The DNA Biotechnology Facility offers the following services:
- DNA Sequencing and Fragment Analysis
- DNA Isolation
- Supply Center Program
Two levels of DNA Sequencing services are available:
- Comprehensive "Full Service"
- Economical "Run-Only Service"
Typical turnaround time for DNA sequencing is approximately 24 hours. The Facility houses two capillary electrophoresis-based DNA sequencers for sequencing:
- Applied Biosystems 3130XL Genetic Analyzer
- Beckman Coulter CEQ 2000XL DNA Sequencer
DNA Fragment Analysis is offered with the Beckman Coulter CEQ sequencer only.
The Facility offers a DNA Isolation service through the use of a QIAGEN BioRobotic System. This service is designed for users who isolate plasmid or M13 phage DNA in large numbers of samples. Service completion time is about three hours per 96-well plate sample batch. A minimum batch of 96 samples is highly recommended.
Instrumentation is offered as a fee-based program for researchers to access shared equipment. The Facility provides maintenance for the instruments. Available instrumentation includes:
- X-ray Film Developer
- UV/VIS (340-750nm) Micro-plate Reader
- Freeze-drying System
- Bio-Rad 2-D Protein Gel Analysis (protein separation and analysis)
The Bio-Rad 2-D Protein Gel Analysis System consists of an electrophoresis unit with an IEF cell and a PAGE box for multiple gel separation of proteins.
The Facility also offers a Supply Center Program with products from five major vendors:
- Applied Biosystems (ABI)
- New England BioLabs (NEB)
University of Connecticut researchers can order items from any of the vendors through the Program. Regular oligo nucleotide synthesis is ordered with Invitrogen. Modified oligo nucleotide synthesis can be ordered from both Invitrogen and ABI. Shipping and handling charges are waived or absorbed by the Program. Discounts, including promotions, are passed on to end users. The Facility charges a flat recharge fee of 7% on the cost base for each item ordered.
Training and Education
Staff provide training in the use of the Facility’s instruments. Consultations are available for DNA sequencing, sequencing data analysis, and database searches. These services are free, but prospective users must schedule an appointment prior to a consulting session.
Use of the Facility
A principal investigator must register with the Facility before accessing available services. Registration is a simple procedure that records valid account and contact information.
Service orders can be placed by phone, fax, email, or in person. However, orders for oligo DNA/RNA synthesis are taken by email only. DNA sequencing samples are dropped off to the Facility or sent in by mail. Customers are notified when their items and/or samples are ready to be picked up.
Facility Use Fees:
Pricing for DNA sequencing and fragment analysis services can be found on the DNA Biotechnology Facility’s website: http://www.biotech.uconn.edu/dbf/pdf/dbf-feeschedule.pdf.
Please contact the Facility Head for specific Supply Center Program prices.
All rates are subject to periodic review and change.